Home >> ALL ISSUES >> 2016 Issues >> Next-gen sequencing workflow in full spate

Next-gen sequencing workflow in full spate

Print Friendly, PDF & Email

William Check, PhD

April 2016—With next-generation sequencing’s clear benefits—for diagnosis, prognosis, treatment, and trials—come its new challenges, and clinical laboratories are doing what it takes and sharing how. Two plenary speakers at last year’s meeting of the Association for Molecular Pathology spoke of variant calling in the bioinformatic pipeline and validation, and of clinical reporting. Colin Pritchard, MD, PhD, of the University of Washington and one of the speakers, sees reporting a genomic sequencing assay as more like making a histologic diagnosis, which he calls craftwork, than reporting a sodium value. “That’s an idea that hasn’t really permeated yet,” he said.

In a separate AMP workshop, Brian Shirts, MD, PhD, presented an expanded view of clinical reporting in his talk on communicating genomic information to physicians. Dr. Shirts is working with two large collaborative groups funded by the National Human Genome Research Institute to improve reporting. “Our long-term goal is to influence providers of electronic health records and policymakers to improve the presentation of clinical genetic information,” Dr. Shirts, an assistant professor in the Department of Laboratory Medicine at the University of Washington, said in an interview.

“The EMR is not just an electronic version of the paper record,” Dr. Shirts said. “It should do something more than just be a file system for bunches of documents.”

Results of an exploratory survey of 17 medical institutions made clear that there is no consensus at this time about where genetic information should go in the electronic health record or how it should be displayed. Dr. Shirts described the situation as “chaos.” Recommendations have been made; figuring out how best to implement them will take considerable further work.

The three presenters spoke recently with CAP TODAY.

Dr. Berger

Dr. Berger

Next-generation sequencing is performed as highly focused “hotspot” panels, large gene panels, and whole exome sequencing. (See “In next-gen sequencing, panel versus exome,” CAP TODAY, January 2016, page 1). Michael F. Berger, PhD, and his colleagues in the Department of Pathology at Memorial Sloan Kettering Cancer Center have settled on a midsize cancer gene panel that detects 5,770 protein coding regions of 410 genes and 46 introns, which they call Memorial Sloan Kettering-Integrated Mutation Profiling of Actionable Cancer Targets, or MSK-IMPACT (Cheng DT, et al. J Mol Diagn. 2015;17:251–264). He reported in the plenary session the results of a large clinical evaluation of the panel.

“Hotspot panels are appropriate for some institutions only doing clinical applications,” Dr. Berger told CAP TODAY. Their advantages are shorter TAT and smaller amount of DNA required. They could be less costly because they would require smaller sequencers.

“It all depends on your throughput,” said Dr. Berger, associate director of the Marie-Josée and Henry R. Kravis Center for Molecular Oncology. He and colleagues perform panels now for more than 500 patients per month; the goal is 10,000 to 15,000 cases per year. “With many samples like we are doing and investing in larger sequencers, the cost difference [between a hotspot panel and a larger gene panel] is not so great. And we can generate a lot more data per sample at a lower cost per base pair.”

The midsize cancer gene panel is scalable and more easily identifies copy number alterations and rearrangements because it involves exon capture by hybridization, not PCR amplification. And the panel’s greater depth of coverage gives them greater power to detect low frequency and subclonal mutations.

impactWorkflow

To validate MSK-IMPACT, Dr. Berger’s laboratory adhered to New York State Department of Health regulations. They observed each variant in 10 positive control samples, using hundreds of samples that had been sequenced in other clinical tests in their laboratory. In addition, they reported results across many runs and technologists.

In the MSK-IMPACT Clinical Sequencing Initiative, the laboratory focused on patients with recurrent or metastatic cancer because they are the patients most likely to be helped. Results were used to provide diagnostic and prognostic information, to select targeted therapies, and to screen patients for clinical trial eligibility.

The billable cancers were lung, colon, melanoma, thyroid, and gastrointestinal stromal tumors, all of which made up much less than half of the samples investigated. “The majority fell into the ‘nonbillable’ category,” Dr. Berger said. “We are absorbing the cost to find out more about patient profiles.”

A meaningful result was obtained on 84 percent of about 8,000 samples. Among these 6,800 cases, breast, colorectal, and non-small cell lung cancers were the most frequent. In all, they sequenced more than 60 general tumor types and more than 300 specific tumor types.

A median of four mutations were found per sample (mean was seven), which means that some samples had many mutations. “When you have so many mutations, most are passengers,” Dr. Berger said. In these instances, prior cases can offer guidance. At MSKCC they use cBioPortal, a software tool developed in-house that can be accessed via any Internet browser and contains results from more than 8,000 cases. Noting which variants have been observed in many other cancer cases can make it easier to identify likely driver mutations.

Among the 6,800 successfully sequenced DNA samples, the most frequently altered cancer genes were TP53, TERT, KRAS, PIK3CA, and APC.

Dr. Berger addressed the conundrum of germline mutations. “We have avoided returning germline results” when seeking variants in cancer genes. “Initially,” he said, “intentional germline analysis was not performed. Only results found unintentionally during quality control were returned. We do a lot of careful QC for every run.”

“One thing we do is to look for evidence of tumor in the normal tissue. We look at copy number of normal diploid cells. Occasionally there might be an inherited copy number alteration. In those instances, because patients were signing a consent form, there was discussion if and how those unintentional germline findings should be returned.”

New York state approved MSK-IMPACT as a germline test in May 2015. From that time on, patients were able to opt in to simultaneous germline testing after viewing an instructional video and providing additional consent. Eighty-two genes in MSK-IMPACT are associated with cancer predisposition.

Anonymized analysis of the first 1,570 cases in which germline analysis was performed revealed that 13 percent had one (or more) pathogenic or likely pathogenic variant. What was unexpected was that only 81 of 198 (41 percent) presented in canonical tumor types. For example, only 28 of 52 (54 percent) of BRCA1/2 variants were found in breast, ovary, prostate, or pancreas cancer, which Dr. Berger calls “surprising and a little bit confusing.” These mutations presented also in patients with no family history.

While cBioPortal acts as a source of genomic information for clinicians, it can also work in the reverse direction. Clinicians can put relevant clinical information into the patient rec-ord, which will allow clinical and genomic correlations to be discovered. “Crowdsourcing among physicians at Memorial is hard to do,” Dr. Berger said. “This part of the project will require collaboration with other places.”

Another ongoing downstream study seeks outcomes data from MSK-IMPACT results. It aims to answer these questions:

  • How many cases harbor actionable mutations?
  • In how many cases is management changed based on molecular alterations?
  • Do mutations correlate with outcomes or response to therapy?
  • Are responses modulated by co-mutational patterns?
  • Is tumor heterogeneity related to response?

Dr. Pritchard, associate director of UW Medicine’s clinical genetics and solid tumors laboratory, showed a slide in his talk of a Boeing 747 towing a child on a tricycle. The caption: “Too much power?” “Our ability to sequence the genome greatly outstrips our ability to understand genomics,” he said.

“Right now in the clinical space the sweet spot is for targeted panels that focus narrowly on a few hundred genes. With these panels you can maximize the quality of the data you get and still be able to interpret the gene variations you find within the scope of disease you are considering.”

Dr. Pritchard presented the pros and cons of buying versus building the software infrastructure necessary for next-gen sequencing. His group came down on the side of building. (See “IT staffing considerations for the NGS laboratory,” CAP TODAY, February 2016, page 77.)

To be able to detect all forms of variants, from single nucleotide variants to deletions and rearrangements, Dr. Pritchard recommends parallelization—running the data through several variant callers in parallel. “Variant callers can have different strengths,” he said. “Stacking multiple approaches increases sensitivity.” This is especially important for structural variants. As with all assays, however, increasing sensitivity lowers specificity. One approach is to use multiple variant callers with different performance characteristics in parallel along with sophisticated manual review to minimize reporting of false-positive results. “This has been very important and successful for us,” Dr. Pritchard said.

Using this strategy, he and his colleagues developed the pipeline UW-OncoPlex, which provides simultaneous deep-sequencing information, based on greater than 500× average coverage, for all classes of mutations in 262 clinically relevant genes (Pritchard CC, et al. J Mol Diagn. 2014;16:56–67). In validation studies, UW-OncoPlex correctly identified 129 of 130 known mutations.

When adding targets to the panel, revalidation is essential. As with the initial validation, it is important to use both dry and wet controls and manual and automated processes. “When thinking about revalidating a pipeline, you want to run both DNA samples with known certain variants and data sets that have previously been called with the validated version of the pipeline.” The results should be evaluated with automated processes and manual review by experts who are familiar with the data. “I still want to lay eyes on it,” Dr. Pritchard said of manual review.

With NGS, coverage varies across the genome. “We need ways to flag low-coverage areas,” he said. A level of unacceptable coverage needs to be defined. While a sample might have average coverage of several hundred times, local coverage at the exon level might be much lower in some regions.

For further accuracy, each laboratory should establish an internal variant database, Dr. Pritchard recommends. This is a database, maintained at the institution, in which all variants detected in that laboratory are stored, along with interpretations. “In talking to my colleagues it seems like people are doing this,” he said. “It is not published so much, but molecular pathologists recognize it as important.”

An “error profile” is also a crucial database to maintain. Dr. Pritchard’s group is running 10 large capture-based sequencing panels for cancer as well as assays for inherited diseases. “It is critical to have an error profile on each assay,” he said.

Also integral to any pipeline will be custom genotyping. As an example, Dr. Pritchard displayed a screen shot of essentially raw data showing the variant MSH2c.942+3A>T, a common pathogenic Lynch syndrome mutation often missed by conventional variant callers because it is at the end of a string of A’s in the genome. “All platforms have a tough time with variants at the end of a homopolymer run,” Dr. Pritchard said. So his group built in a custom feature that says, “Tell me how many T’s there are at this position no matter what.” That flags the area for director review.

“In general,” Dr. Pritchard suggests, “anything that is really clinically actionable you may want to flag for analytical review plus expert review.”

To illustrate that special analyses can be done with customized NGS, Dr. Pritchard showed analysis by NGS of specimens for microsatellite instability (Hempelmann JA, et al. J Mol Diagn. 2015;17:705–714; Salipante SJ, et al. Clin Chem. 2014;60:1192–1199). “Directly determined MSI with NGS may be better than with capillary electrophoresis,” he said. Microsatellite instability has traditionally been done with capillary electrophoresis plus fragment ana-lysis, and that method remains predominant. “I think increasingly it is being recognized that MSI can be very accurately determined with NGS,” he said. “However, the bioinformatics have to be done appropriately.”

Adapted from J Mol Diagn. Vol.16, Pritchard CC, et al. “Validation and implementation of targeted capture and sequencing for the detection of actionable mutation, copy number variation, and gene rearrangement in clinical cancer specimens,” 56–57. ©Elsevier (2014).

Adapted from J Mol Diagn. Vol.16, Pritchard CC, et al. “Validation and implementation of targeted capture and sequencing for the detection of actionable mutation, copy number variation, and gene rearrangement in clinical cancer specimens,” 56–57. ©Elsevier (2014).

Of the clinical reporting of NGS results, Dr. Pritchard said it requires more than simply pushing a button. “Bioinformatics alone will not get us a correct diagnosis most of the time,” he said.

“Particularly among my basic research colleagues, although they know more about scripting, sometimes they miss the point of what is happening in the clinical lab. In reporting there needs to be a balance between how much is automated and how much the laboratory director reviews.

“Without bioinformatics there is no way we could do this,” he continued. “But I don’t think we could do this at all without expert people doing manual review. And I don’t think this is going to change anytime soon. We need to understand the patient and the subtleties of the data itself.”

Dr. Pritchard

Dr. Pritchard

Interpreting the pathogenicity of variants requires use of databases of known variants. “One of the best external databases is ClinVar, where you can review the evidence base for germline variants,” Dr. Pritchard said. Another good browser is ExAC, a database of variants from more than 60,000 cancer patients (exac.broadinstitute.org) established by the Exome Aggregation Consortium. “It is important to understand what ExAC is and is not,” Dr. Pritchard said. For instance, it does not include all types of variations, particularly not copy number alterations.

Sign-out in the UW genetics and solid tumors laboratory employs a multidirector, multidisciplinary model. To start, at least two reviewers select independently a short list of variants, which a review board then looks at. “We’re certainly not the only ones taking this approach,” Dr. Pritchard said. “Many centers doing large panels have a physician board review of one sort or another. It’s been a successful strategy for us. For every NGS panel we do, at least two people review the data, and often more”—typically four or five. One director writes the final report.

Of the cost of this approach, he said: “Local tumor boards enable true personalized medicine.” They’re good, too, for residents and fellows in pathology and laboratory medicine, who “almost universally love it.”

Dr. Shirts is a member of a large National Human Genome Research Institute-funded consortium called Clinical Sequencing Exploratory Research, consisting of 377 researchers from 21 institutions. It was established to guide the dissemination and implementation of best practices for integrating sequencing into clinical care. Dr. Shirts has been part of a working group that is looking at how exome and genome results are displayed in the electronic health record. In the exploratory survey of 17 institutions, they found that within the same hospital, genomic information can be displayed in many different formats: free text, structured data, physician notes. Moreover, the same molecular result can be in two formats depending on whether it was found in an exome or targeted gene test.

Molecular results can go in different places. Pathology reports usually go into a separate tab. Most institutions don’t have a structure for genetic results to go into a separate place where they can be queried quickly. Results were predominantly displayed as PDF documents without decision support (Tarczy-Hornoch P, et al. Genet Med. 2013;15:
824–832).

In an interview, Dr. Shirts addressed the question of decision support for genetic information.

“Imagine a test done several years ago, ordered by a different provider but pertinent to what the patient is experiencing now,” he said. “Electronic decision support could be aware of the past medical record and bring it to the attention of the physician. In order for the EHR to behave that way, someone needs to program that in, and in a way that is machine readable.

“One of the major priorities of our group is to move things in the direction that we can have electronic decision support remind physicians of what genetic information is telling them they should be doing for the patient.” Dr. Shirts noted one big difference between genetic information and other tests. “Once a person has a genetic test done, they wouldn’t have it done again. It could stay in the EMR for decades. But physicians look for the most recent results. So they might not see the outcome of a genetic test done a long time ago.”

Collaborating with another National Human Genome Research Institute working group, Electronic Medical Records and Genomics (eMERGE) Network, Dr. Shirts’ group came up with several recommendations, most centered around improved electronic decision support. Here are the three highest ranked recommendations, with the percent of respondents ranking each in their top four (Shirts B, et al. J Am Med Inform Assoc. 2015;22:
1231–1242):

  • Provide clinical decision support for genetic results that are medically actionable (90 percent).
  • Develop mechanisms to trigger an alert about drug interactions if a relevant drug is predicted (70 percent).
  • Develop a mechanism for medically actionable genetic information to trigger an alert to the treating clinician (70 percent).

At this point the recommendations have only the force of persuasion, and that’s all right with Dr. Shirts. “There is currently no regulatory organization for this, and I don’t think we want one right now,” he said.

Dr. Shirts’ EHR working group is now lending its support to the action collaborative DIGITizE (Displaying and Integrating Genetic Information Through the EHR), formed under the auspices of the National Academies. DIGITizE is composed of people from industry, academia, and health care systems. Its goal is to create implementation guides for genomic computerized decision support. “We need to move the field forward a bit more before regulatory work would be beneficial,” Dr. Shirts said. To have FDA regulate NGS right now “could potentially be a disaster for the field,” in Dr. Shirts’ view.

“We do want to have more structures and more standards,” he said, “but we are still trying to figure out what standards make sense.”
[hr]

William Check is a writer in Ft. Lauderdale, Fla.

x

Check Also

With CMS coverage policy, NGS cancer testing goes large

July 2018—The March 16 announcement of a new Centers for Medicare and Medicaid Services coverage policy for next-​generation-sequencing–based diagnostic lab tests for patients with advanced cancer did not appear out of the blue, since a draft policy was issued last fall.

X