May 2016—BD mouse T-lymphocyte and B-lymphocyte kits, protocols, and software templates for the BD Accuri C6 flow cytometer aim to simplify the identification of mouse T and B lymphocytes, their subsets, and their activation markers. The kits include fluorescent antibodies, buffer systems, and controls needed for acquisition and analysis. The panels are compatible with other markers to further characterize cell subpopulations. BD Accuri C6 software templates matched to each kit include predefined workspaces, markers, regions, gates, and parameter names for quick and easy setup and analysis.
BD offers two kits for each type of mouse lymphocyte. The subset antibody cocktail identifies mouse T-cell or B-cell subpopulations. Identification of T lymphocytes is based on expression of CD3, CD4, and CD8a, while characterization of developing and peripheral mouse B lymphocytes is based on expression of CD45R/B220, CD23, and sIgM. The activation antibody cocktail enables rapid characterization of activated mouse T or B cells using the markers CD25 and CD69s.