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Molecular Pathology Selected Abstracts, 7/14

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Molecular pathology abstracts editors: Editors: Donna E. Hansel, MD, PhD, chief, Division of Anatomic Pathology, and professor, Department of Pathology, University of California, San Diego; John A. Thorson, MD, PhD, associate professor of pathology, director of the clinical genomics laboratory, Center for Advanced Laboratory Medicine, UCSD; Sarah S. Murray, PhD, associate professor, Department of Pathology, and director of genomic technologies, Center for Advanced Laboratory Medicine, UCSD; and James Solomon, MD, PhD, resident, Department of Pathology, UCSD.

Is there a role for molecular diagnostics in bladder cancer?

Bladder cancer is the fourth most common cancer in men in the United States and is associated with high rates of cancer morbidity and mortality worldwide. Multiple subtypes of bladder cancer have been identified, the most common of which is urothelial cancer. Once diagnosed, treatment depends on the pathologic grade and stage of the bladder tumor. For superficial tumors, therapy includes transurethral resection and instillation of chemotherapeutic or proinflammatory agents directly into the bladder. However, for tumors that deeply invade the wall of the bladder, patients undergo radical cystectomy and receive chemotherapy if metastases are present, although outcomes for this population remain relatively poor. In attempts to improve therapy, several recent efforts have been undertaken to better characterize molecular alterations associated with bladder cancer and to use this information to develop targeted molecular therapies. Recently, the Cancer Genome Atlas project examined 131 cases of muscle-invasive urothelial carcinoma and analyzed data on whole genome and exome DNA sequencing, DNA copy number, complete mRNA and microRNA expression, DNA methylation, and protein expression and phosphorylation. Mutations were consistently found in 32 genes, including p53 (mutated in 49 percent of bladder tumors), PIK3CA (20 percent), RB1 (13 percent), FGFR3 (12 percent), and TSC1 (eight percent). Integrating these data, the investigators identified major pathways that are often dysregulated in muscle-invasive bladder cancer. Alterations in the p53/Rb pathway, including mutations in CDKN1A and CDKN2A, occurred in 93 percent of tumors. Additional altered pathways that affected cell proliferation and survival included the PI3K/Akt/mTOR and RTK/RAS signaling cascades in the majority of tumors. Several proteins associated with these pathways, including FGFR3, ERBB2 (HER2), EGFR, and ERBB3, are particularly amenable to targeted molecular therapies. Finally, the study identified epigenetic changes in bladder cancer. Chromatin remodeling and histone modification was affected in 89 percent of bladder tumors, more than in any other cancer studied, and the SWI/SNF complex that regulates gene expression was affected in 64 percent of tumors. Overall, this study found that 69 percent of the bladder tumors examined had potential molecular therapeutic targets. It is likely that the findings of this study will help to elucidate the molecular pathogenesis of bladder cancer and will be especially useful as clinical trials are developed to examine molecular therapies in urothelial carcinoma.

The Cancer Genome Atlas Research Network. Comprehensive molecular characterization of urothelial bladder carcinoma. Nature. 2014; 507:315–322.

Correspondence: John N. Weinstein, Seth Lerner, or David J. Kwiatkowski at www.nature.com/nature/journal/v507/n7492/full/nature12965.html#corres-auth

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