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Illumina, MiniSeq

Illumina, MiniSeq

Next-generation sequencing instruments, December 2017

Contact Information

Illumina
info@illumina.com
5200 Illumina Way
San Diego, CA 92122
858-202-4500


Name of instrument MiniSeq
Name of model/Model has been upgraded —/no
Country where designed/Manufactured/FDA cleared or approved U.S./Singapore/not required
First year sold in U.S./Outside U.S./First year installed 2016/2016/2016
Dimensions in inches (H × W × D)/Footprint of all instrumentation and computing hardware 20.4 × 18 × 18.9/2.4 square feet
Equipment supplied with system/Automation for library preparation system is a single unit inclusive of amplification, paired-end sequencing, and analysis/yes
Necessary equipment not included with system and additional cost
Bioinformatics tools provided/For use by biologist or bioinformatician Local Run Manager/biologist; BaseSpace/biologist
Supplied with UPS/Entire workflow can occur in same lab no/yes
Clean room requirements/Electrical connection —/100–240 VAC at 50–60 Hz
List price/Total list price for equipment needed to perform simplest and fastest workflow from amplification through variant calling (not typically found in lab) $49,500/$49,500
Purchase options purchase, reagent rental, or lease
Warranties offered first year included with instrument purchase, extended options available
Training included/Total time for standard install and basic training yes/<1 days
Training location/Follow-up training available on site/yes, extra charge
Instrument core performance:
Maximum No. of libraries amplified in single amplification event 384 for some applications
Read length/Percent bases >Q30 up to 2 × 150 bp/80
Paired-end capability/Tag lengths/Spans yes/up to 350 bp/2 × 150 bp
Fragment/Tag lengths/Spans yes/up to 350 bp/2 × 150 bp
Mate-pair/Tag lengths/Spans yes/up to 350 bp/2 × 150 bp
Single-end/Tag lengths/Spans yes/up to 350 bp/2 × 150 bp
RNA sequencing/Tag lengths/Spans yes/up to 350 bp/2 × 150 bp
ChIP sequencing/Tag lengths/Spans yes/up to 350 bp/2 × 150 bp
Bisulfite sequencing/Tag lengths/Spans yes/up to 350 bp/2 × 150 bp
Output per run up to 7.5 Gb
Total time from library construction to variant calling to achieve output per run/Technical bench time/Bioinformatics time 4–24 hours depending on read length/2 hours/2 hours
Sample preparation:
Total time for generating standard gDNA library <2 hours
• Paired-end <2 hours
• Fragment <2 hours
• Mate-pair <2 days
• Single-end <2 hours
• RNA sequencing <9 hours
• ChIP sequencing <9 hours
• Bisulfite sequencing 5 hours
Hands-on time each:
• Paired-end 15 minutes
• Fragment <3 hours
• Mate-pair <2 hours
• Single-end 15 minutes
• RNA sequencing <5.5 hours
• ChIP sequencing <5.5 hours
• Bisulfite sequencing 3 hours
Equipment required for library construction standard lab equipment
Reagents and controls:
Cost per run $550–$1,545
Cost per sample*
Reagent tracking method on instrument RFID
Information contained in tracking method serial number, expiration date, lot and part numbers, number of cycles, PE-SR
Reagent shipping conditions/Storage conditions box 1: dry ice; box 2: gel pack/box 1: -15°– -20°C; box 2: 2°–8°C
Shelf life of amplification and sequencing reagents guaranteed 3 months
Controls introduced during creation of library/Sequencing control avail. yes/yes
Capable of complete walkaway automation for amplification, sequencing, and variant calling yes
Remote system monitoring yes
Instrument control software and devices to start run/for data analysis Local Run Manager/Local Run Manager
Total time required for setup of amplification, sequencing, and variant calling steps 10 minutes
Maximum No. of libraries sequenced in a single run 384
Types of maintenance plans available parts only, basic, comprehensive, advantage, dedicated on site
No. of field apps scientists and engineers based in U.S.
Weekly maintenance required/Monthly/Pre-run wash/wash/wash
System offers secondary analysis software developed by instrument vendor yes
Variant report generated directly on instrument yes
Third-party analysis software available yes, BaseSpace apps store
Ability of software to detect mutations substitutions, indels, copy number changes
Total No. of peer-reviewed publications for this platform 8 publications total
Published applications in pathology-related research
Fastest published turnaround time from sample to analyzed result
Lowest published variant sensitivity level
Distinguishing features of sequencer (supplied by company) smallest benchtop sequencer available; based on industry’s most-adopted sequencing technology; single instrument goes from prepared library through paired-end sequencing and analysis with no ancillary equipment
*inclusive of sample prep, for multiple configurations, using 1 Mb and 30x average coverage per sample
Note: a dash in lieu of an answer means company did not answer question or question is not applicable
All information is supplied by the companies listed. The tabulation does not represent an endorsement by the CAP.