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Illumina, MiniSeq

Illumina, MiniSeq

Next-generation sequencing instruments, December 2017

Contact Information

5200 Illumina Way
San Diego, CA 92122

Name of instrument MiniSeq
Name of model/Model has been upgraded —/no
Country where designed/Manufactured/FDA cleared or approved U.S./Singapore/not required
First year sold in U.S./Outside U.S./First year installed 2016/2016/2016
Dimensions in inches (H × W × D)/Footprint of all instrumentation and computing hardware 20.4 × 18 × 18.9/2.4 square feet
Equipment supplied with system/Automation for library preparation system is a single unit inclusive of amplification, paired-end sequencing, and analysis/yes
Necessary equipment not included with system and additional cost
Bioinformatics tools provided/For use by biologist or bioinformatician Local Run Manager/biologist; BaseSpace/biologist
Supplied with UPS/Entire workflow can occur in same lab no/yes
Clean room requirements/Electrical connection —/100–240 VAC at 50–60 Hz
List price/Total list price for equipment needed to perform simplest and fastest workflow from amplification through variant calling (not typically found in lab) $49,500/$49,500
Purchase options purchase, reagent rental, or lease
Warranties offered first year included with instrument purchase, extended options available
Training included/Total time for standard install and basic training yes/<1 days
Training location/Follow-up training available on site/yes, extra charge
Instrument core performance:
Maximum No. of libraries amplified in single amplification event 384 for some applications
Read length/Percent bases >Q30 up to 2 × 150 bp/80
Paired-end capability/Tag lengths/Spans yes/up to 350 bp/2 × 150 bp
Fragment/Tag lengths/Spans yes/up to 350 bp/2 × 150 bp
Mate-pair/Tag lengths/Spans yes/up to 350 bp/2 × 150 bp
Single-end/Tag lengths/Spans yes/up to 350 bp/2 × 150 bp
RNA sequencing/Tag lengths/Spans yes/up to 350 bp/2 × 150 bp
ChIP sequencing/Tag lengths/Spans yes/up to 350 bp/2 × 150 bp
Bisulfite sequencing/Tag lengths/Spans yes/up to 350 bp/2 × 150 bp
Output per run up to 7.5 Gb
Total time from library construction to variant calling to achieve output per run/Technical bench time/Bioinformatics time 4–24 hours depending on read length/2 hours/2 hours
Sample preparation:
Total time for generating standard gDNA library <2 hours
• Paired-end <2 hours
• Fragment <2 hours
• Mate-pair <2 days
• Single-end <2 hours
• RNA sequencing <9 hours
• ChIP sequencing <9 hours
• Bisulfite sequencing 5 hours
Hands-on time each:
• Paired-end 15 minutes
• Fragment <3 hours
• Mate-pair <2 hours
• Single-end 15 minutes
• RNA sequencing <5.5 hours
• ChIP sequencing <5.5 hours
• Bisulfite sequencing 3 hours
Equipment required for library construction standard lab equipment
Reagents and controls:
Cost per run $550–$1,545
Cost per sample*
Reagent tracking method on instrument RFID
Information contained in tracking method serial number, expiration date, lot and part numbers, number of cycles, PE-SR
Reagent shipping conditions/Storage conditions box 1: dry ice; box 2: gel pack/box 1: -15°– -20°C; box 2: 2°–8°C
Shelf life of amplification and sequencing reagents guaranteed 3 months
Controls introduced during creation of library/Sequencing control avail. yes/yes
Capable of complete walkaway automation for amplification, sequencing, and variant calling yes
Remote system monitoring yes
Instrument control software and devices to start run/for data analysis Local Run Manager/Local Run Manager
Total time required for setup of amplification, sequencing, and variant calling steps 10 minutes
Maximum No. of libraries sequenced in a single run 384
Types of maintenance plans available parts only, basic, comprehensive, advantage, dedicated on site
No. of field apps scientists and engineers based in U.S.
Weekly maintenance required/Monthly/Pre-run wash/wash/wash
System offers secondary analysis software developed by instrument vendor yes
Variant report generated directly on instrument yes
Third-party analysis software available yes, BaseSpace apps store
Ability of software to detect mutations substitutions, indels, copy number changes
Total No. of peer-reviewed publications for this platform 8 publications total
Published applications in pathology-related research
Fastest published turnaround time from sample to analyzed result
Lowest published variant sensitivity level
Distinguishing features of sequencer (supplied by company) smallest benchtop sequencer available; based on industry’s most-adopted sequencing technology; single instrument goes from prepared library through paired-end sequencing and analysis with no ancillary equipment
*inclusive of sample prep, for multiple configurations, using 1 Mb and 30x average coverage per sample
Note: a dash in lieu of an answer means company did not answer question or question is not applicable
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