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Illumina, NextSeq 550Dx Instrument

Illumina, NextSeq 550Dx Instrument

Next-generation sequencing instruments, December 2017

Contact Information

5200 Illumina Way
San Diego, CA 92122

Name of instrument NextSeq 550Dx Instrument
Name of model/Model has been upgraded —/no
Country where designed/Manufactured/FDA cleared or approved U.S./Singapore/yes
First year sold in U.S./Outside U.S./First year installed 2017/2017/2017
Dimensions in inches (H × W × D)/Footprint of all instrumentation and computing hardware 23 × 21 × 27/4 square feet
Equipment supplied with system/Automation for library preparation system is inclusive for flowcell clustering, sequencing, and data analysis; library preparation is done off board/yes
Necessary equipment not included with system and additional cost general laboratory supplies (variable)
Bioinformatics tools provided/For use by biologist or bioinformatician Local Run Manager/biologist
Supplied with UPS/Entire workflow can occur in same lab yes (extra charge)/yes
Clean room requirements/Electrical connection —/100–120 VAC 15 A or 220–240 VAC 10 A
List price/Total list price for equipment needed to perform simplest and fastest workflow from amplification through variant calling (not typically found in lab) $347,000/$347,000
Purchase options purchase, reagent rental, lease
Warranties offered first year included, extended options available
Training included/Total time for standard install and basic training yes/1 day install, 2 days training
Training location/Follow-up training available on site/yes, extra charge
Instrument core performance:
Maximum No. of libraries amplified in single amplification event 384 for some applications
Read length/Percent bases >Q30 up to 150 bp/75
Paired-end capability/Tag lengths/Spans yes/up to 550 bp/up to 2 × 150 bp
Fragment/Tag lengths/Spans yes/up to 550 bp/up to 2 × 150 bp
Mate-pair/Tag lengths/Spans yes/2–12 kb/up to 2 × 150 bp
Single-end/Tag lengths/Spans yes/up to 550 bp/up to 2 × 150 bp
RNA sequencing/Tag lengths/Spans yes/up to 550 bp/up to 2 × 150 bp
ChIP sequencing/Tag lengths/Spans yes/up to 550 bp/up to 2 × 150 bp
Bisulfite sequencing/Tag lengths/Spans yes/up to 550 bp/up to 2 × 150 bp
Output per run up to 120 Gb
Total time from library construction to variant calling to achieve output per run/Technical bench time/Bioinformatics time 22–45 hours depending on application/90 minutes–12 hours/ 30 minutes–12 hours
Sample preparation:
Total time for generating standard gDNA library <2 hours
• Paired-end <2 hours
• Fragment <2 hours
• Mate-pair <2 days
• Single-end <2 hours
• RNA sequencing <9 hours
• ChIP sequencing <9 hours
• Bisulfite sequencing 5 hours
Hands-on time each:
• Paired-end 15 minutes
• Fragment <3 hours
• Mate-pair <2 hours
• Single-end 15 minutes
• RNA sequencing <5.5 hours
• ChIP sequencing <5.5 hours
• Bisulfite sequencing 3 hours
Equipment required for library construction standard lab equipment
Reagents and controls:
Cost per run $1,060–$5,562
Cost per sample*
Reagent tracking method on instrument RFID
Information contained in tracking method serial number, expiration date, lot and part numbers, number of cycles
Reagent shipping conditions/Storage conditions dry ice, gel pack, or ambient/-20°C, 4°C, 25°C
Shelf life of amplification and sequencing reagents guaranteed 3 months
Controls introduced during creation of library/Sequencing control avail. yes/yes
Capable of complete walkaway automation for amplification, sequencing, and variant calling yes
Remote system monitoring yes
Instrument control software and devices to start run/for data analysis 10 minutes/5 minutes
Total time required for setup of amplification, sequencing, and variant calling steps 35 minutes
Maximum No. of libraries sequenced in a single run up to 384
Types of maintenance plans available parts only, basic, comprehensive, advantage plan
No. of field apps scientists and engineers based in U.S.
Weekly maintenance required/Monthly/Pre-run none/manual wash if instrument is idle for 2 weeks/none, automatic wash is completed after every run
System offers secondary analysis software developed by instrument vendor yes
Variant report generated directly on instrument yes
Third-party analysis software available yes
Ability of software to detect mutations substitutions, indels, copy number changes
Total No. of peer-reviewed publications for this platform >11,000 publications using Illumina sequencing chemistry
Published applications in pathology-related research
Fastest published turnaround time from sample to analyzed result
Lowest published variant sensitivity level
Distinguishing features of sequencer (supplied by company) FDA-regulated NGS platform allows clinical diagnostics in Dx mode and clinical research applications in research mode; FDA-regulated content includes NextSeq 550Dx High-Output Reagent Kit v2, TruSeq Custom Amplicon Kit Dx, and FFPE QC Kit; flexible output for sample multiplexing and different research applications, from targeted panels to whole genome
*inclusive of sample prep, for multiple configurations, using 1 Mb and 30x average coverage per sample
Note: a dash in lieu of an answer means company did not answer question or question is not applicable
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